Cellular Internalization of Nano β-Tricalcium Phosphate via Arf6 Associated Endocytosis and Its Bone Regeneration Potential
| dc.contributor | Pusat kanser Tun Abdullah Ahmad Badawi, Universiti Sains Malaysia | |
| dc.contributor.author | Wan Syafiqa Meor Hissan | |
| dc.date.accessioned | 2026-04-10T03:10:44Z | |
| dc.date.available | 2026-04-10T03:10:44Z | |
| dc.description | This dataset investigates the cellular internalization and bone regeneration potential of nano β-tricalcium phosphate (nβ-TCP) as a bone graft material. The synthesized nβ-TCP consists of irregular nanorods (~300 nm agglomerates, –10 mV ζ-potential) and was confirmed as phase-pure β-TCP using XRD and FTIR. In vitro, MC3T3-E1 pre-osteoblasts treated with nβ-TCP (100–500 µg/mL) showed high viability (~80–90%), indicating low cytotoxicity. TEM imaging and protein analysis reveal that nβ-TCP is internalized via an actin-dependent, Arf6-associated, clathrin-independent endocytic pathway, supported by inhibition studies using cytochalasin D, amiloride, and nystatin. In vivo, nβ-TCP and commercial β-TCP were fabricated into pellets and implanted in rabbit tibia. nβ-TCP demonstrated higher mechanical strength (~6.5 MPa vs. ~3.1 MPa) and favorable biological response, with preserved cortical structure and reduced fibrotic changes after 13 weeks. Overall, the dataset provides novel insight into nano-biomaterial uptake mechanisms and supports the potential of nβ-TCP as a biocompatible and effective bone graft material. | |
| dc.identifier | FRGS/1/202/STG05/USM/02/2 | |
| dc.identifier.uri | https://opendata.usm.my/handle/123456789/74736 | |
| dc.language.iso | en | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Cellular Internalization of Nano β-Tricalcium Phosphate via Arf6 Associated Endocytosis and Its Bone Regeneration Potential | |
| dc.type | Image | |
| oaire.fundingStream | Ministry of Higher Education Malaysia for Fundamental Research Grant Scheme |
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