Complete chloroplast genome data for Cryptocoryne elliptica (Araceae) from Peninsular Malaysia
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Date
2022-03-23
Authors
Nurul Shakina Mohd Talkah
Suwidji Wongso
Ahmad Sofiman Othman
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The aquatic plant genus Cryptocoryne, a popular plant genus in the aquarium industry, is made up of more than 50 described species and some 15 naturally occurring named and unnamed interspecific hybrids. Cryptocoryne elliptica has a restricted distribution in the north part of Peninsular Malaysia. Destruction of its natural habitats for various human activities has led to a decline in numbers. Here, we report the complete chloroplast genome of C. elliptica and establish a molecular dataset for a maternally inherited genome. Here, we utilized an Illumina NovaSeq 6000 protocol to sequence the partial genome of C. elliptica and used bioinformatic tools to reconstruct the chloroplast genome in de novo mode. The assembled chloroplast genome is a circular DNA molecule 159,968 bp in length. The chloroplast genome has a quadripartite structure composed of a large single-copy region of 96,273 bp and a small single-copy (SSC) region of 15,205 bp, separated by a pair of inverted repeats (IRa and IRb), each of which is 24,245 bp. The chloroplast genome of C. elliptica encodes a total of 108 genes, comprising 74 protein-coding genes, 30 tRNA genes and 4 rRNA genes. In total, 204 SSR loci were identified, most of which were located within intergenic regions.
Description
High-quality reads were assembled using NOVOPlasty 4.2.1 and Novowrap 1.12. The assembled scaffold was annotated using GeSeq. Exon and intron position was confirmed using blastn function from NCBI Nucleotide Blast. The circular chloroplast genome map was drawn using OGDRAW.
Link to data: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA664218