A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1

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dc.contributorUniversiti Sains Malaysia
dc.contributorUniversiti Sains Malaysia
dc.contributorUniversiti Sains Malaysia
dc.contributorUniversiti Sains Malaysia
dc.contributorUniversiti Sains Malaysia
dc.contributorUniversiti Malaysia Sabah
dc.contributor.authorSharvin Manickam
dc.contributor.authorShallinie Thangadurai
dc.contributor.authorAzali Azlan
dc.contributor.authorZarina Amin
dc.contributor.authorGhows Azzam
dc.contributor.authorMardani Abdul Halim
dc.date.accessioned2023-05-10T08:16:19Z
dc.date.available2023-05-10T08:16:19Z
dc.date.issued2022-11-13
dc.descriptionTotal RNA was extracted from 3-5 days old adult fly control testes and testes with miR-2b-1 overexpression using Trizol and Qiagen RNeasy mini kit. These samples were sequenced, and transcriptome were analyzed. Link to data: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE211399
dc.description.abstractMicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that negatively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is conserved in invertebrates. miRNAs play key roles in gene regulation, cell proliferation, cell death, cell differentiation and cell developmental homeostasis in multicellular organisms. Its role in various human diseases is continuously being studied. miRNAs also found out to be crucial in maintaining stem cell niche in D. melanogaster gonads. We have identified that ectopic overexpression of miR-2b-1 of D. melanogaster causes testicular bulging (a tumour like phenotype) in 3-5 days old adult flies. Hence, we have performed a transcriptomic (RNA-seq) analysis to understand the role of miR-2b-1 in the development, maintenance, and differentiation of D. melanogaster adult testis stem cells. Data are available from GEO (accession number GSE211399).
dc.identifier.doi10.1016/j.dib.2022.108748
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE211399
dc.identifier.urihttps://opendata.usm.my/handle/123456789/74642
dc.publisherElsevier
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.titleA transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1
dc.typeDataset
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Biological, Chemical, and Mathematical Sciences