Transcriptome dataset from bark and latex tissues of three Hevea brasiliensis clones
No Thumbnail Available
Date
2020-08-17
Authors
Mohd Fahmi Abu Bakar
Urwashi Kamerkar
Siti Nurfazilah Abdul Rahman
Muhd Khairul Luqman Muhd Sakaff
Ahmad Sofiman Othman
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Hevea brasiliensis is exploited for its latex production, and it is the only viable source of natural rubber worldwide. The demand for natural rubber remains high due its high-quality properties, which synthetic rubber cannot compete with. In this paper, we present transcriptomic data and analysis of three H. brasiliensis clones using tissue from latex and bark tissues collected from 10-year-old plant. The combined, assembled transcripts were mapped onto an H. brasiliensis draft genome. Gene ontology analysis showed that the most abundant transcripts related to molecular functions, followed by biological processes and cellular components. Simple sequence repeats (SSR) and single nucleotide polymorphisms (SNP) were also identified, and these can be useful for selection of parental and new clones in a breeding program. Data generated by RNA sequencing were deposited in the NCBI public repository under accession number PRJNA629890.
Description
Three rubber clones namely RRIM 600, PB 260 and RRIM 929 were used in this study, each with a low, medium, and high latex yield. From these clones, bark and latex tissue was collected from 10-year-old plants, with three replicates collected for each sample. Transcriptome analysis was performed using cDNA libraries of H. brasiliensis. The assembled contigs were also used for DEG analysis, as well as SNPs and SSRs marker discovery.
Link to data: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA629890