Metagenomic 16S rDNA amplicon data of microbial diversity of Cimex hemipterus (F.) (Hemiptera: Cimicidae) treated with insect growth regulators (IGR)
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Date
2023-04
Authors
NurHidayah Taibukahn
Abdul Hafiz Ab Majid
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Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The metagenomics dataset presented here is based on bacterial 16S rDNA gene amplicons of DNA extracted from tropical bed bugs (Cimex hemipterus). Amplicon-based sequencing was performed using the Illumina MiSeq platform, and the raw sequence data were analyzed using QIIME (version 2022.8.3). The metagenome sequence comprised ten samples that include C1 (133 511bps), C2 (108 920bps), CH1 (106 562bps), CH2 (101 778bps), P1 (103 618bps), P2 (133 258bps), T1 (113 558bps), T2 (133 952bps), TM1 (125 335bps), and TM2 (118 345bps). The sequence data is readily accessible at the NCBI SRA under bio project PRJNA918835. The most abundant microbial community present in the C. hemipterus is the Proteobacteria, with more than 99% of the abundance.
Description
The HiYield TM Genomic DNA isolation kit (Real Biotech Corporation, Taiwan) was used to recover the microbial DNA from fully-fed tropical bed bugs that had been crushed. Illumina MiSeq platform was used to carry out 16S v3-v4 amplicon metagenomics sequencing.
URL to data: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA918835/